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Reasoning for slow off-kinetics of luciferase in yeast light-switch system

This inference chain connects incomplete Pfr-Pr conversion, persistent Pr-PhyA:FHY1/FHL interaction, and the modeled Pr_FHL intermediate with the experimentally observed slow decline of luciferase signal after far-red treatment. The logic is partially supported by simulations and highlights uncertainty regarding the exact properties of the intermediate state.

Confidence
80%
◑partialactivecomplexity: mid

Reasoning Steps (3)

Incomplete conversion to Pr form leaves residual active PfrStep 1
Pr form PhyA and FHY1/FHL residual interaction slows off-kineticsStep 2
Properties of Pr_FHL intermediate state remain undeterminedStep 3

Source

Synthesis for current paper

Connections (4)

Residual interaction of Pr form PhyA and FHY1/FHL explains slow off-kineticsAssociation
Far-red exposure produces incomplete Pr conversion; residual Pfr persistsAssociation
Pr_FHL intermediate state crucial for system kinetics in modelAssociation
Stability of Luc reporter affects but does not fully explain off-kineticsAssociation