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Spaceflight alters plant gene expression

Claim that exposure to spaceflight induces changes in gene expression in Arabidopsis seedlings and undifferentiated cell cultures.

Confidence
90%
active

Evidence Quote

“Spaceflight induces changes in gene expression in seedlings and undifferentiated cultured Arabidopsis cells.”

Relationship

spaceflight condition alters gene expression

Arguments

Space environment stress factorssubject
Auxin metabolismobject

Connections (18)

Spaceflight triggers adaptive plant gene expression through novel environmental perceptionInferenceChain
Spaceflight alters plant gene expressionAssociation
Validated multi-step workflow for gene expression analysis during spaceflightInferenceChain
Experiment design ensures detection of spaceflight-induced gene expression changesInferenceChain
hsfA2 heat shock mutantFactor
Stress response and detoxification genes expression changes in Arabidopsis under microgravityFactor
Senescence metabolism genesFactor
Terpenoid metabolism genesFactor
Seed metabolism genesFactor
Spaceflight alters expression of stress and regulatory genes in Arabidopsis cellsInferenceChain
HSP17.6A gene (cell cultures)Factor
AGP12 gene (cell cultures)Factor
Spaceflight induces organism-wide changes in gene expressionInferenceChain
Spaceflight triggers divergent molecular responses in seedlings vs. cultured cellsInferenceChain
External experiments support generalizability of plant and animal gene regulation themesInferenceChain
Environmental stress regulates transcription factors in ArabidopsisInferenceChain
Spaceflight and abiotic stress impact plant gene expression via conserved molecular pathwaysInferenceChain
Cross-study reasoning on plant gene expression adaptations in spaceflightInferenceChain

Evidence

“Figure 2 displays thermal profiles of spaceflight and ground control BRIC units across the experiment timeline, with launch, fixation, and landing points marked. Temperature data collection started after sealing, showing most temperatures between 21.7ºC and 24.8ºC until fixation on orbit.”

How does gamma radiation affect DNA methylation in Arabidopsis?

“Both qRT-PCR and SAGE confirmed trends of differential gene expression observed in the microarray analysis for representative up- and downregulated genes in seedlings and cell cultures (e.g., MLP423, MAPKKK13, HSP17.6A, AGP12) exposed to spaceflight versus ground control.”

Anna-Lisa Paul et al. (2005). Arabidopsis Spaceflight Transcriptomes doi:10.1089/ast.2005.1.44 ↗

“SAGE analysis supported microarray data by confirming that heat shock–related genes are activated in Arabidopsis cultured cells during spaceflight, as indicated by the scatter plot in Figure 5 and corresponding SAGE and microarray fold-change comparisons in Figure 7.”

Anna-Lisa Paul et al. (2005). Arabidopsis Spaceflight Transcriptomes doi:10.1089/ast.2005.1.44 ↗

“Allen et al. (2009) report gene expression changes in mouse skeletal muscle following spaceflight.”

Allen, D.L., Bandstra, E.R., Harrison, B.C., Thorng, S., Stodieck, L.S., Kostenuik, P.J., Morony, S., Lacey, D.L., Hammond, T.G., Leinwand L.L., Argraves, W.S., Bateman, T.A., and Barth, J.L. (2009) Effects of spaceflight on murine skeletal muscle gene expression. J Appl Physiol 106:582–595.

“Branco-Price et al. (2005) present genome-wide transcript and translation changes in Arabidopsis seedlings under hypoxia.”

Branco-Price, C., Kawaguchi, R., Ferreira, R.B., and Bailey-Serres, J. (2005) Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation. Ann Bot 96:647–660.