Red light increases luciferase expression
Red light input to the circuit increases luciferase output through PhyA/FHL action on the GAL1 promoter in yeast.
Evidence Quote
“Red light induces rapid increase of luminescence in yeast patches expressing GAL1:LUC.”
Relationship
Connections (5)
Evidence
“Empirical results showing red/far-red light pulses yield switchable luciferase output in yeast with GAL1:LUC reporter and PhyA/FHL, PhyA/FHY1, or PhyB/PIF3 fusion proteins. Multiple panels detail responses to different light protocols and chromophore conditions.”
“Evidence that yeast can activate light-switchable phytochrome system in the absence of exogenously supplied PCB chromophore; induction occurs but is reduced compared to with PCB.”
“Experimental comparison shows PhyA-FHY1 (and PhyA-FHL) pairs give higher luciferase induction and lower background versus PhyB-PIF3 in synthetic yeast gene expression system.”
“Modeling of phytochrome-driven luciferase activation in yeast; fit to dynamic luminescence timeseries from luciferase imaging experiments. Incorporates substrate diffusion, photoconversion, complex sequestration and intermediate states.”
“Mathematical model includes spatial aspects of agar cultures, fitting initial substrate diffusion regime and matching empirical luminescence responses across variable yeast patches.”
“ODE model predicts and experiments confirm that it takes ~100 hours for complete removal of transcriptional activation after red light stimulus in the synthetic yeast system under specified conditions.”
“Model simulations predict that short intervals between red and far-red light pulses increase transcription rate, while longer intervals create baseline oscillations. Enables protocol design for desired gene expression dynamics.”
“Experiments and model show that introducing an intermediate state during Pfr_FHL photoconversion is essential to capture slow switching-off of luciferase expression in response to light change.”
“Evidence describing the use of MOTA tool to reduce model parameters from 17 non-identifiable to 10 identifiable parameters by fixing some parameters.”
“Empirical data showing luminescence levels from yeast patches with gene expression system under different light protocols and luciferin pretreatment conditions.”
“Data showing basal expression of GAL1:LUC reporter in yeast patches lacking PHYA, PHYB, FHY1, or FHL under red or far-red light and with or without PCB chromophore.”