Association·nasa
Photoactivated phytochrome A regulates luciferase reporter in yeast
Claim that red light converts phytochrome A into photoactivated Pfr form, which binds FHY1/FHL fusion to induce transcription of luciferase gene in yeast; far-red light reverses this activation.
Confidence
90%
active
Evidence Quote
“Red light induces Pfr-PhyA interaction with FHY1/FHL fusion protein, activating luciferase transcription; far-red reverses this.”
Relationship
Phytochrome A photoactivation/deactivation by red/far-red light regulates Luciferase gene expression in yeast
Arguments
Connections (6)
Mechanistic reasoning for red/far-red light-induced gene regulation in yeastInferenceChain
FHL-PhyA complex activates transcription via GAL1 promoterAssociation
Mechanistic reasoning for switchable light-induced luciferase in yeastInferenceChain
Mechanistic reasoning for phytochrome light-switch gene regulationInferenceChain
Mechanistic reasoning for yeast light-switch gene regulationInferenceChain
Mechanistic reasoning for phytochrome light-switch gene regulation in yeastInferenceChain
Evidence
“Experiments and model show that introducing an intermediate state during Pfr_FHL photoconversion is essential to capture slow switching-off of luciferase expression in response to light change.”
“Evidence describing the use of MOTA tool to reduce model parameters from 17 non-identifiable to 10 identifiable parameters by fixing some parameters.”
Synthesis for current paper