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Luciferin substrate diffusion in agar controls initial yeast luminescence decrease — NASA Knowledge Graph

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Luciferin substrate diffusion in agar controls initial yeast luminescence decrease

Claim that the observed initial decrease in luminescence after luciferin addition in yeast is governed primarily by the diffusion coefficient of luciferin through agar gel, modeled using the diffusion equation.

Confidence
80%
active

Evidence Quote

“Initial decrease in luminescence results from diffusion of substrate through agar”

Relationship

Diffusion coefficient of luciferin in agar controls initial decrease Cytosolic luciferin concentration

Arguments

Diffusion coefficient of luciferin in agarsubject
Luciferase gene expression in yeastobject

Connections (4)

Stability of Luc reporter affects but does not fully explain off-kineticsAssociation
Cytosolic luciferin concentrationFactor
Reasoning for substrate diffusion-limited light output in yeast coloniesInferenceChain
Reasoning for robustness and accuracy of gene circuit kinetic model fittingInferenceChain