Association·nasa
Heat shock genes upregulated in cultured cells by spaceflight
Claim that heat shock–associated genes are strongly upregulated in Arabidopsis cultured cells during spaceflight, supported by SAGE and microarray analysis.
Confidence
90%
active
Evidence Quote
“SAGE and microarray confirm activation of heat shock genes in spaceflight cultured cells”
Relationship
spaceflight cultured cells increases heat shock genes
Connections (6)
Spaceflight gene expression changes are confirmed by multiple methods and support adaptive response themeInferenceChain
hsfA2 heat shock mutantFactor
Spaceflight alters plant gene expressionAssociation
Spaceflight triggers divergent molecular responses in seedlings vs. cultured cellsInferenceChain
Environmental stress regulates transcription factors in ArabidopsisInferenceChain
Cross-study reasoning on plant gene expression adaptations in spaceflightInferenceChain
Evidence
“Both qRT-PCR and SAGE confirmed trends of differential gene expression observed in the microarray analysis for representative up- and downregulated genes in seedlings and cell cultures (e.g., MLP423, MAPKKK13, HSP17.6A, AGP12) exposed to spaceflight versus ground control.”
“SAGE analysis supported microarray data by confirming that heat shock–related genes are activated in Arabidopsis cultured cells during spaceflight, as indicated by the scatter plot in Figure 5 and corresponding SAGE and microarray fold-change comparisons in Figure 7.”