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Modification-aware sequencing methods aid detection of modified DNA

Claim that new sequencing technologies (e.g., REMoDE, mEnrich-seq, Nanopore, LC-MS/MS) can support detection and characterization of modified DNA in microbial communities.

Confidence
80%
active

Evidence Quote

“Modification-aware sequencing methods can help detect modified DNA.”

Relationship

Modification-aware sequencing methods enables detection Serratia modif genome

Arguments

Modification-aware sequencing methodssubject
Serratia phage 92A1 arabinosylated genomeobject

Connections (3)

Genome contamination yields HGT false positivesAssociation
Technical workflow enables and validates phage DNA modification discoveryInferenceChain
Detection of DNA/RNA modifications and elusive phage genomes requires specialized enrichment and sequencing methodsInferenceChain

Evidence

“Study describing the REMoDE DNA enrichment method for metagenomic sequencing”

Enam SU et al. (2023). Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing doi:10.1128/aem.01670-22 ↗

“Preprint describing mEnrich-seq for methylation-guided enrichment sequencing in microbiome studies”

Cao L et al. (2022). mEnrich-seq: Methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome doi:10.1101/2022.11.07.515285 ↗

“Evidence demonstrating REMoDE (Restriction Endonuclease-Based Modification-Dependent Enrichment) as a method to enrich DNA based on modification for metagenomic sequencing, as described by Enam et al. (2023).”

Enam SU et al. (2023). Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing doi:10.1128/aem.01670-22 ↗

“Evidence that mEnrich-seq is a methylation-guided enrichment sequencing method for bacterial taxa of interest, as described by Cao et al. (2022).”

Cao L et al. (2022). mEnrich-seq: Methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome doi:10.1101/2022.11.07.515285 ↗