Association·arcadia
DNA modification biases metagenome-based genome recovery
Claim that DNA modification by phages may hinder the recovery or detection of their genomes in community metagenomic sequencing datasets.
Confidence
80%
active
Evidence Quote
“DNA modification may bias or reduce metagenomic recovery of phage genomes.”
Relationship
DNA modification biases metagenome recovery biases Serratia metagenome abund
Arguments
Connections (4)
Explaining low metagenomic detectability of modified phages and need for specialized approachesInferenceChain
Phage U32 peptidase may have novel DNA modification roleAssociation
Technical workflow enables and validates phage DNA modification discoveryInferenceChain
Detection of DNA/RNA modifications and elusive phage genomes requires specialized enrichment and sequencing methodsInferenceChain
Evidence
“Evidence examining phage mechanisms for overcoming bacterial defense systems, as summarized by Samson et al. (2013).”
Samson JE et al. (2013). Revenge of the phages: defeating bacterial defences doi:10.1038/nrmicro3096 ↗
“Evidence describing the determination of the non-sequencable DNA phage YerA41 genome using RNA-sequencing, as detailed by Leskinen et al. (2020).”
Leskinen K et al. (2020). YerA41, a Yersinia ruckeri Bacteriophage: Determination of a Non-Sequencable DNA Bacteriophage Genome via RNA-Sequencing doi:10.3390/v12060620 ↗