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Circular DNA produces large insert sizes

Circularized DNA creates insert size distributions with unexpectedly large mapped distances between paired reads.

Confidence
100%
active

Evidence Quote

“Circular DNA creates paired reads with mapped distances far longer than expected (~300 bp), resulting in large insert sizes.”

Relationship

Circular DNA increases Mapped read insert size

Arguments

Circular DNAsubject
Mapped read insert sizeobject

Connections (4)

Reasoning linking circular DNA characteristics to detection via short-read mapping workflowInferenceChain
de Bruijn graph compaction reduces memory usageAssociation
How circular DNA properties enable detection by short-read mappingInferenceChain
Linking circular DNA properties to short-read detection methodsInferenceChain

Evidence

“nf-core circdna pipeline for extrachromosomal circular DNA detection.”

(2024). circdna link ↗

“ecc_finder: A robust and accurate tool for detecting extrachromosomal circular DNA.”

Zhang P et al. (2021). ecc_finder: A Robust and Accurate Tool for Detecting Extrachromosomal Circular DNA From Sequencing Data doi:10.3389/fpls.2021.743742 ↗

“Circlehunter: a tool to identify extrachromosomal circular DNA from ATAC-Seq data.”

Yang M et al. (2023). Circlehunter: a tool to identify extrachromosomal circular DNA from ATAC-Seq data doi:10.1038/s41389-023-00476-0 ↗

“eccDNA-pipe: an integrated pipeline for identification, analysis, and visualization of extrachromosomal circular DNA.”

Fang M et al. (2024). eccDNA-pipe: an integrated pipeline for identification, analysis and visualization of extrachromosomal circular DNA from high-throughput sequencing data doi:10.1093/bib/bbae034 ↗

“Extrachromosomal circular DNA is common in yeast.”

Møller HD et al. (2015). Extrachromosomal circular DNA is common in yeast doi:10.1073/pnas.1508825112 ↗

“Endogenous circular DNA (eccDNA) in Caenorhabditis elegans and Homo sapiens.”

Shoura MJ et al. (2017). Intricate and Cell Type-Specific Populations of Endogenous Circular DNA (eccDNA) in Caenorhabditis elegans and Homo sapiens doi:10.1534/g3.117.300141 ↗

“FLED: a full-length eccDNA detector for long-reads sequencing data.”

Li F et al. (2023). FLED: a full-length eccDNA detector for long-reads sequencing data doi:10.1093/bib/bbad388 ↗

“ATAC-seq identifies thousands of extrachromosomal circular DNA in cancer and cell lines.”

Kumar P et al. (2020). ATAC-seq identifies thousands of extrachromosomal circular DNA in cancer and cell lines doi:10.1126/sciadv.aba2489 ↗

“Reference describing Minimap2 software for nucleotide sequence alignment”

Li H (2018). Minimap2: pairwise alignment for nucleotide sequences doi:10.1093/bioinformatics/bty191 ↗

“Fast and accurate short read alignment with Burrows–Wheeler transform.”

(2009). Li & Durbin 2009 on BWA doi:10.1093/bioinformatics/btp324 ↗

“The Sequence Alignment/Map format and SAMtools.”

(2009). Li et al. 2009 on SAMtools doi:10.1093/bioinformatics/btp352 ↗

“BEDTools: a flexible suite of utilities for comparing genomic features.”

(2010). Quinlan & Hall 2010 on BEDTools doi:10.1093/bioinformatics/btq033 ↗

“Reference to the fastp tool for FASTQ data preprocessing”

(2018). fastp: an ultra-fast all-in-one FASTQ preprocessor doi:10.1093/bioinformatics/bty560 ↗

“NCBI SRA Tools for sequence data processing.”

sra-tools