Association·nasa

Procedural controls validate glycomics from RNA column debris

Procedural control using A. thaliana xyloglucan mutants and RNA column-derived debris indicates that glycome profiling using root debris from RNA purification columns with one-step 4 M KOH extraction can reliably report environmentally-induced cell wall changes.

Confidence
80%
active

Evidence Quote

“Glycome profiling of plant debris from RNA purification columns using one extraction step can still reliably report environmentally-induced cell wall changes.”

Relationship

Glycome profiling from RNA column root debris enables Environmentally-induced cell wall changes

Arguments

Glycome profiling from RNA column root debrissubject
Environmentally-induced cell wall changesobject

Connections (2)

Procedural controls and multimodal validation for spaceflight-induced cell wall changesInferenceChain
Xyloglucan mAbs bind to root microsomal fractionsAssociation

Evidence

“Lu et al. (2017) identify epigenome modifications at single-base resolution in upland cotton (Gossypium hirsutum L.) corresponding to drought stress response.”

Lu, X. & et al. (2017). Single-base resolution methylomes of upland cotton (Gossypium hirsutum L.) reveal epigenome modifications in response to drought stress link ↗

“Li et al. (2017) present a genome-wide comparative study showing distinct DNA methylation patterns between soybean cytoplasmic male-sterile line NJCMS5A and its maintainer NJCMS5B.”

Li, Y. & et al. (2017). Genome-wide comparative analysis of DNA methylation between soybean cytoplasmic male-sterile line NJCMS5A and its maintainer NJCMS5B link ↗