Association·nasa

Batch effects impact DEG identification

Claim that batch effects and confounding variables affect identification of differentially expressed genes (DEGs) across spaceflight transcriptome experiments.

Confidence
100%
active

Evidence Quote

Batch effects became evident and impact DEGs across studies

Relationship

batch effects affects DEGs

Evidence

Method for batch-effect correction in RNA-seq data

Fei, T. & Yu, T. (2020). ScBatch: Batch-effect correction of RNA-seq data through sample distance matrix adjustment link

Study on confounding factors in RNAseq analysis of rodent spaceflight experiments

Lai Polo, S.-H. & et al. (2020). RNAseq analysis of rodent spaceflight experiments is confounded by sample collection techniques link

Comparison of different software packages for detecting differential expression in RNA-seq data

Seyednasrollah, F. et al. (2015). Comparison of software packages for detecting differential expression in RNA-seq studies link