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Microchamber confinement enhances cell imaging and observation

Use of the 3D-printed microchamber device increases the proportion of cells in focus and allows robust imaging of motile and nonmotile cells, relative to standard 384-well plate.

Confidence
90%
active

Evidence Quote

“The microchamber confined a higher proportion of Colpoda steinii cells for observation, improving imaging compared to the 384-well plate.”

Relationship

3D-printed microchamber device increases Continuous observation of cells

Arguments

3D-printed microchamber devicesubject
Continuous observation of cellsobject

Connections (3)

Microchamber design enables robust imaging and preserves cell behaviorInferenceChain
Microchamber components enable microscopyAssociation
Reasoning chain: Microchamber assembly improves cell observation and preserves behaviorInferenceChain

Evidence

“Reference for Fiji software used in image analysis.”

Schindelin J et al. (2012). Fiji: an open-source platform for biological-image analysis doi:10.1038/nmeth.2019 ↗

“Reference for CellProfiler 4 software for image analysis.”

Stirling DR et al. (2021). CellProfiler 4: improvements in speed, utility and usability doi:10.1186/s12859-021-04344-9 ↗

“Reference for the R statistical computing environment.”

R Core Team (2021). R: A language and environment for statistical computing link ↗