Methodology and empirical evidence support for pain and itch detection

This reasoning chain evaluates the methodological and empirical factors, including references to mesoscale connectome studies and in vivo calcium imaging, that underpin claims about the ability (and limitations) of label-free autofluorescence neuroimaging and related optical approaches to detect pain- and itch-related neural activity in mouse brain. It integrates evidence on experimental sensitivity, mechanistic behavior, and the context of supporting imaging technologies.

Reasoning Steps (2)

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