Factor·arcadia

HPLC analysis of RNA and contaminating DNA nucleosides

HPLC chromatograms showing RNA from E. coli and E. coli infected with phage T4 before and after rRNA depletion, revealing DNA nucleosides co-eluting with purified mRNA complicating detection of modifications.

Confidence
100%
active

Source

Phage mRNA chemical analysis via HPLC and RNA-seq